Cyanine5 hydrazide是用于标记醛和酮的活性染料。是Cy5® hydrazide的类似物。 该染料能够很容易与含羰基的分子以近乎理想化的程度反应。比如承受氧化压力的蛋白,糖基化的蛋白通过高碘酸氧化作用重新活化(包括抗体),含醛基的寡核苷酸。 Cyanine5 hydrazide可以替代carbonyl-reactive Cy5®, Alexa Fluor 647, DyLight 649等染料。
Cy® is a trademark of GE Healthcare.
General properties
Appearance:
dark blue powder
Molecular weight:
569.61
Molecular formula:
C32H42Cl2N4O
Solubility:
significant solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)
Quality control:
NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:
Download
Spectral properties
Excitation maximum, nm:
646
Extinction coefficient at excitation maximum, Lmol-1cm-1:
250000
Emission maximum, nm:
662
Fluorescence quantum yield:
0.2
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