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Human serum characterized for the hemolytic function of individual complement proteins

The Quidel Specimen Stabilizing Solution (A9576), when used according to the procedure described in this data sheet, will retard the generation of complement activation fragments and complexes in human serum, plasma and other specimens during processing and storage. This product extends the stability of the specimen, significantly reducing the likelihood of in vitro -generated elevation of complement fragments in clinical specimens. This product should be used for stabilizing all serum and plasma samples which cannot be stored at –70°C or below within four hours of phlebotomy. The Quidel specimen stabilizing solution can be used with all assays designed to determine the concentration of complement fragments and complexes in human serum and plasma, specifically the MicroVue iC3b EIA Kit, C3a EIA Kit, Bb EIA Kit C4d EIA Kit, and SC5b-9 EIA Kit.

Stabilized specimens may be stored for up to six days at 4?C prior to testing or at –20?C or below for one month. Storage of unstabilized specimens at –20?C or warmer will result in complement activation.

Stabilized specimens should be tested as soon after thawing as possible. As with any complement specimen, thaw at 37?C. Remove to ice as soon as the specimen has thawed. Do not leave specimens at 37?C. Since the specimen is already diluted 1:2 in specimen stabilizer, the required assay dilution should be reduced two-fold (e.g., if the required dilution is 1:50, make a 1:25 of the stabilized specimen). Normal ranges should be determined for stabilized specimens independently. In some assays, the concentration of the analyte will differ slightly between stabilized and unstabilized specimens.