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19.6 kDa

Gene was obtained from mouse DNA expressed in E. coli modified as described in Day, et al. (1992)
"Engineered disulfide bond greatly increases specific activity of recombinant murine interferon beta" ( J. Interferon Res. 12: 139-43)

> 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg

Measured using a cytopathic effect inhibition assay on Mouse (L929) cells with EMCV. EC50 for IFNβ is ~ 2.5 U/ml

20 mM HEPES, pH 6.0; 0.5M NaCl; 6% Glycerol

< 1 EU/μg

A combination of ion exchange, hydrophobic interaction and size exclusion chromatography

For retention of full activity store at -70 oC or below and avoid repeated freeze-thaw cycles