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19.1 kDa

cDNA obtained from mouse leukocyte interferon expressed in E.coli

> 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg

Measured using a cytopathic inhibition assay on Mouse (L929) cells with EMCV.
The EC50 for IFN in this assay is ~ 5 U/ml.

Phosphate-buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)

< 1 EU/μg

A combination of ion exchange, hydrophobic interaction and size exclusion chromatography

For retention of full activity store at -70 oC or below and avoid repeated freeze-thaw cycles