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Beta-Hydroxybutyric Acid (bHB) ELISA Kit

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Beta-Hydroxybutyric Acid (bHB) ELISA Kit

品牌：Biomatik

货号：EKU02686

规格：96T

货期：

应用：

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Beta-Hydroxybutyric Acid (bHB) ELISA Kit

商品详情参考文献相关资料

Species Reactivity	Multispecies (General)
Format	96 well plate
Detection Range	1.23-100 μg/mL
Sensitivity	0.48 μg/mL
Sample Type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Time	2 hours
Gene Name	Beta-Hydroxybutyric Acid
Gene Aliases	OHB; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid
Application	ELISA
Assay Type	Competitive
Detection Method	Colorimetric
Shipping Condition	Blue ice
Storage Instructions	-20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Precaution Of Use	The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Expiry Date	8 months
Specificity	This assay has high sensitivity and excellent specificity for detection of Beta-Hydroxybutyric Acid (bHB). No significant cross-reactivity or interference between Beta-Hydroxybutyric Acid (bHB) and analogues was observed.
Test Principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Beta-Hydroxybutyric Acid (bHB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Beta-Hydroxybutyric Acid (bHB) and unlabeled Beta-Hydroxybutyric Acid (bHB) (Standards or samples) with the pre-coated antibody specific to Beta-Hydroxybutyric Acid (bHB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-Hydroxybutyric Acid (bHB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Beta-Hydroxybutyric Acid (bHB) in the sample.
Assay Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Hydroxybutyric Acid (bHB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions	For research use only. Not for diagnostic procedures.
Quality Systems	The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research Area	Signal transduction;Metabolic pathway;Endocrinology;