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产品介绍
品名	Rabbit anti-Tescalcin
货号	T876
规格	200ul
克隆性	polyclonal
共轭	rabbit
反应性	nonconjugated
宿主动物	Rabbit
抗体形式	Antiserum lyophil.
产品形式	Lyophilized whole serum (no preservatives)
复溶	with 200 μl of bi-distilled water.
产品描述	The antiserum against Tescalcin is produced in rabbits by immunization with recombinant mouse Tescalcin containing a 6-his tag at the N-terminal. The antibody was evaluated for specificity and potency: a) by Biotin-Avidin labeling of cryostate-, vibratome- and paraffin-sections of 4% paraformaldehyde fixed brains and b) by immunoblots (Girard et al, 2015).
产品用途	Tescalcin is a novel calcineurin B-like protein that binds a single Ca2+ ion. It occurs mainly in the heart; stomach and brain (see also the Allen brain atlas (http://www.brainmap.org/).
稀释	Immunohistochemistry: 1:2'000 - 1:5'000, on paraformaldehyde (4%) or formalin-fixed tissue, cryostate or paraffin-sections.  Immunoblots: 1:1'000 - 1:2'000. For immunohistochemistry and immunoblots the titre was determined by using the avidin-biotin method.  We recommend that the optimal dilutions be determined by titration experiments.
储存	After reconstitution freeze in small aliquots (e.g. 1 μl) and keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
参考文献	Girard F, Venail J, Schwaller B, Celio MR. (2015) The EF-hand Ca(2+)-binding protein super-family: a genome-wide analysis of gene expression patterns in the a dult mouse brain.Neuroscience;294:116-55.