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Ca2+-ATPase 1 (human plasma membrane)
品牌:Swant
货号:PMCA1
规格:PMCA1
货期:swant

品牌:Swant 货号:PMCA1 品名:Ca2+-ATPase 1 (human plasma membrane) 规格:200?μl

商品详情 参考文献 相关资料

产品介绍

品名

Rabbit anti-Ca2+-ATPase, isoform 1

货号

PMCA1

规格

200 μl

克隆性 

polyclonal

共轭

nonconjugated

反应性

human

宿主动物

Rabbit

抗体形式

Antiserum lyophil

产品形式

Lyophilized whole serum (no preservatives)

重构

with 200 μl of bidistilled water

产品描述

The antiserum against Ca2+-ATPase, isoform 1, was produced in rabbits by immunization with the N-terminal sequence-specific peptide of the human pump as epitope. It recognizes the corresponding proteins in human, rat and mouse tissue. The antibody was evaluated for specificity and potency : a) by Biotin-Avidin labelling of cryostatesections of 4% paraformaldehyde fixed tissue and b) by immunoenzymatic labelling of Western blots. It can also be used for immunoprecipitation. The serum was inactivated at 50°C for 30 minutes

稀释

Immunohistochemistry : 1: 1'000 - 1: 2'000. 

Immunoblots : 1: 1'000 - 1: 2'000. 

For immunohistochemistry and immunoblots the titer was determined by using the avidin-biotin method. We recommend that the optimal dilutions be determined by titration experiments

储存

After reconstitution freeze in small aliquots (e.g. 1 μl) and keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing

参考文献

1. Stauffer T.P., Guerini D., Carafoli E. (1995) Tissue distribution of the four gene products of the plasma-membrane Ca2+-pump. A study using specific antibodies. J. Biol. Chem. 19: 12184- 12190. 

2. Stauffer T.P., Guerini D., Celio M.R., Carafoli E. (1997) Immunolocalization of the plasma membrane Ca2+-pump isoforms in the rat brain. Brain Res. 748:21-29.


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