品牌:Emfret
货号:M110-1
品名:Emfret FITC-labeled Rat Anti-Mouse CD9 (p24)
规格:1.5 ml / 300 tests
同种型:Nyn.H3 / Rat (Wistar) IgG2a
包含:FITC-labeled immunoglobulin in 20 mM Tris buffer with 137 mM NaCl, 0.5%
BSA and 0.09% (w/v) sodium azide
特异性:
The Nyn.H3 antibody reacts with murine CD9 (p24), a 24-kDa single chain surface
glycoprotein that is a member of the tetraspanin family1,2. CD9 is expressed on platelets and many
leukocytes and has a broad tissue distribution. CD9 is involved in cell adhesion, cell migration, and
integrin signaling.
用途:
The antibody preparation is optimized for flow cytometric applications: Use 5 μl to stain ~106
platelets or ~0.5x106 cells in a recommended volume of 25 μl. Incubate for 15 minutes at room
temperature, stop reaction by addition of 400 μl PBS and analyze samples within 30 minutes.
For immunofluorescent staining of acetone-fixed frozen sections, the appropriate dilution must be
determined individually.
制备和储存:
The antibody was purified from hybridoma cell culture supernatant by
Protein G-Sepharose chromatography. The antibody was conjugated with FITC under optimum
conditions. The solution is free of unbound FITC. Store product undiluted at 4°C and avoid prolonged
exposure to light. Stable for one year from date of shipment. Do not freeze.
注意:
Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations
containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may
be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free
buffer.
注:仅供研究使用,不用于诊断或治疗用途。