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3 hours

50 μL (consult the Package Insert for dilution instructions for all sample types)

 For Research Use Only. Not for use in diagnostic procedures.

 The MicroVue Complement Multiplex assays measures the amount of various complement proteins in human plasma or serum specimens. Choose between available off-the-shelf assays or have a custom kit developed with the specific analytes required (see Ordering Information below for more information).

The included analytes are available in two panels:

• Panel 1: Ba, Bb, C2 Intact, C3a, C3d, C4a, C4d, C5a, sC5b-9, Factor D, Factor H, Factor I
• Panel 2: C1q, C2 Intact, C3 Intact, C4 Intact, C5 Intact, Factor D, Factor P

 Certain analytes cannot be combined into the same custom multiplex assays due to cross-reactivity. Incompatible analytes include:

• Panel 1: C3a and C3d
• Panel 1: C4a and C4d

The Complement System consists of more than 50 fluid and membrane-bound proteins and protein fragments organized into four primary pathways: the Classical, Alternative, Lectin and Terminal. Through specific activation steps complement proteins mediate a set of activities including inflammation, clearance of immune complexes, disruption of cell membranes, and regulation of the immune response. Deficiencies in the complement cascade can predispose individuals to infection through ineffective opsonization or defects in the ability to lyse invading pathogens. Also, the complement system is associated with many acute and chronic diseases due to abnormalities in its function. Quantitative analysis of different complement proteins and their activation products are of great importance in experimental and clinical medicine. The ability to measure multiple complement proteins in an individual sample constitutes a major step forward in research-based tools. The MicroVue Complement Multiplex can quantitively measure twelve individual complement proteins, providing a comprehensive overview of complement system homeostasis and activation.

Sample volume per well (50 μL) 

Sample Dilution 

Antigen incubation time (2 hours) 

Wash wells (3X) 

Detection volume per well (50 μL) 

Detection incubation time (1 hour) 

Wash wells (3X) 

SHRP 1X volume per well (50 μL) 

SHRP 1X incubation time (20 minutes) 

Wash wells (6X) 

Substrate volume per well (50 μL) 

Imaging Time (250-300 sec)