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human, mouse

immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section, immunohistochemistry - free floating section

Rabbit

Antiserum lyophil.

Lyophilized antiserum (no preservatives).

with 200 μl / 500 μl / 1 ml bi-distilled water (depending on the volume you ordered). 

This antiserum was produced against recombinant rat parvalbumin. It cross-reacts with many other species, humans included. It can be used in immunohistochemistry  and for immunoblotting.

Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method. 

Immunoblots: 1:500 - 1:1'000 

We recommend that the optimal dilutions be determined by titration experiments.

Reconstitute with 200 μl bi-distilled water and make small portions upon arrival (e.g. 2-5 μl). For long storage, keep at - 80°C (or at least - 20°C). For continuous use keep the diluted antiserum at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

1. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press 

2. Celio M.R., Heizmann C.W. (1981) Nature 293: 300-302 

3. Celio M.R., Heizmann C.W. (1982) Nature 297:504-506 

4. Schwaller B., et al. (1999) Am. J. Physiol. 276. C395-403 

5. *Filice F, Celio M.R., Szabolcsi V. (2017) JCN, in press