BioAimScientific 1010011 人血小板衍生的生长因子BB快速ELISA试剂盒 人血小板衍生的生长因子BB试剂盒 Human PDGF-BB EasyTest? ELISA Kit
Human PDGF-BB EasyTest? ELISA KitHomeELISAEasyTest? ELISA KitsHuman PDGF-BB EasyTest? ELISA Kit
Human PDGF-BB EasyTest? ELISA Kit
Human PDGF-BB EasyTest ELISA Kit Standard Curve
Human PDGF-BB EasyTest? ELISA Kit
Catalog No : 1010011
Sizes
$529.00
Product Features
Product Name Human PDGF-BB EasyTest? ELISA Kit
Assay Type Solid Phase Sandwich ELISA
Format 96-well strip plate
Assay Process One wash step, two incubations
Assay Length 1.5 hours
Sample Volume Serum , Plasma, 50μL
Detection Method Colorimetric
Sensitivity 6 pg/mL
Assay Range 31.3-2000pg/mL
Reactivity Human
Cross-Reactivity No cross-reactivity observed with available related molecules
Gene Names PDGFB; IBGC5; PDGF-2; PDGF2; SIS, SSV, c-sis
Gene ID 5155
Swissprot ID P01127
Recovery
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Serum
|
80
|
73-83
|
|
Plasma
|
82
|
75-87
|
Linearity
|
Sample Type
|
Dilution
|
% of Expected
|
|
Serum
|
1:2
|
100
|
|
1:4
|
102
|
|
1:8
|
126
|
|
Plasma
|
1:2
|
99
|
|
1:4
|
98
|
|
1:8
|
90
|
Standard Curve: Human PDGF-BB EasyTest? ELISA Kit
Kit Components
? Pre-Coated 96-well Strip Microplate
? Wash Buffer
? Stop Solution
? Assay Diluent
? Standards
? Bioaim human PDGF-BB mix
? TMB One-Step Substrate
Protocol Outline
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 50 μl of standard or sample to each well.
3. Add 50 μl of Bioaim mix.
4. Incubate 1 h at RT.
5. Wash and add 100 μl of TMB One-Step Substrate Reagent to each well.
6. Incubate 15 min at RT.
7. Add 50 μl of Stop Solution to each well.
8. Read at 450 nm/570 nm.
Background
The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases.
Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor.
Storage
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Regulatory Status
Human PDGF-BB EasyTest? ELISA Kit is for Research Use Only.
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