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GenDEPOT公司 Western Blot预制缓冲液及相关产品目录


用于蛋白质印迹的预制缓冲液产品

1. 样品裂解

类别 产品名称 货号  规格
裂解缓冲液 RIPA Cell Lysis Buffer (1X) with EDTA R4100-010 100 ml
RIPA II Cell Lysis Buffer with Triton (1X), without EDTA R4200-010 100 ml
蛋白酶/磷酸酶
抑制剂混合物溶液 		Xpert Protease Inhibitor Cocktail Solution (100X) P3100-001 1 ml
Xpert Phosphatase Inhibitor Cocktail Solution (100X) P3200-001 1 ml
Xpert Duo Inhibitor Cocktail Solution (100X) P3300-001 1 ml
蛋白琼脂糖 Puredown Protein A-Agarose, BSA-Binding Site Blocked P9201-015 1.5 ml
Puredown Protein G-Agarose, BSA-Binding Site Blocked P9202-015 1.5 ml
Puredown Protein A/G-Agarose, BSA-Binding Site Blocked P9203-015 1.5 ml
蛋白质
定量 		Bradford (Coomassie) Protein Assay Plus Kit P7200-050 Kit
Bicinchoninic Acid (BCA) Protein Assay Kit P8100-050 Kit
蛋白质样品缓冲液 Laemmli Sample Buffer (4X), Reducing L1100-001 10 ml
Laemmli Sample Buffer (4X), Non-Reducing L1200-001 10 ml
Native Sample Buffer (4X) N2300-002 20 ml

                                                               

2. SDS-PAGE 

类别 产品名称 货号                      规格
SDS-PAGE凝胶 Acrylamide : bis 30%, 29:1 Solution A0418-050 495 ml
Acrylamide : bis 40%, 29:1 Solution A0421-050 495 ml
1.5M Tris-HCl Buffer (pH 8.8) T8101-050 500 ml
0.5M Tris-HCl Buffer (pH 6.8) T8102-050 500 ml
10％ SDS Solution S0792-050 500 ml
20％ SDS Solution S0793-050 500 ml  
Ammonium Persulfate (APS) Tablets, 150 mg A7503-020 20 Tablets
TEMED T0512-002 25 ml
运行染色液 Tris, Ultra Pure T9200-100 1 Kg
Glycine, 99％ G0610-100 1 Kg
SDS, 99％ Ultra Pure S0800-100 1 Kg
10X Tris-Glycine SDS buffer (Running buffer) T8053-050 500 ml
蛋白质标志物 Prosi Prestained Protein Marker P8500-040 2 X 200 μl
Xpert Prestained Protein Marker P8502-050 2 X 250 μl
Xpert 2 Prestained Protein Marker P8503-050 2 X 250 μl
凝胶染色 ProsiBlue Gel Staining Solution P7300-050 500 ml


3. (Wet) Transfer

类别 产品名称 货号  规格
转印膜 West-Q PVDF Membrane, Roll, 0.22 um, 270mm x 3M W7031-270 Roll
West-Q PVDF Membrane, Roll, 0.45 um, 270mm x 3M W7032-270 Roll
NitroPure Nitrocellulose Transfer Membrane,Roll, 0.22 um , 300mm x 3M LC7033-300 Roll
NitroPure Nitrocellulose Transfer Membrane,Roll, 0.45 um , 300mm x 3M LC7034-300 Roll
转印缓冲液 10X Tris-Glycine Native Buffer (Transfer buffer) T8052-050 500 ml
膜染色 Ponceau S Solution P9100-010 100 ml


4. Western Blot

类别 产品名称 产品货号  规格

Blocking Albumin, Ultra pure Bovine Serum Albumin A0100-010 100 g
20X TBS Buffer, pH 7.4 T8054-050 500 ml
10X TBST Buffer, pH 7.4 T8056-100 1L
Tween 20, Molecular Biology Grade T9100-010 100 ml
Incubation Case (5 slots) DWG003 1 EA
二抗 Goat anti-Mouse IgG(H+L)-HRP, 1 mg/ml SA001-500 500 μl
Goat anti-Rabbit IgG(H+L)-HRP, 1 mg/ml SA002-500 500 μl
Rabbit anti-Goat IgG(H+L)-HRP, 1 mg/ml SA007-500 500 μl


5. Detection

类别 产品名称 货号       规格


ECL 		West-Q Chemiluminescent Substrate Kit, Plus  W3651-012 120 ml
West-Q Pico ECL Solution W3652-020 200 ml
West-Q Pico Dura ECL Solution  W3653-020 200 ml
West-Q Femto Clean ECL Solution W3680-010 100 ml

West-Q ECL Selection guide

产品名称 货号 产品特点 检测范围
West-Q Chemiluminescent Substrate Plus Kit  W3651 High sensitivity & stable signalsEconomical
price 		Picogram
West-Q Pico ECL Solution  W3652 High sensitivity & stable
signalLow background 		Low Picogram
West-Q Pico Dura ECL Solution W3653 High Sensitivity & Stable SignalExcellent
Signal Persistence 		Low Picogram
West-Q Femto Clean ECL Solution  W3680 Very high sensitivity & stable
signalsVery low background 		Femtogram


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